Stabilized protein preparations for a tissue adhesive

US6447774B1 · kind B1 · utility

Assignee

• Aventis Behring GmbH · DE

Inventors

• Hubert Metzner · Marburg, DE
• Peter Gronski · Marburg, DE

Key dates

• Filing date: Jul 13, 2001
• Grant date: Sep 10, 2002
• Expiry date: Jul 13, 2021

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61L24/106
• WIPO field: Medical technology
• WIPO sector: Instruments

Abstract

A tissue adhesive is described, which contains three separate components, i.e.,a stabilized, essentially fibrinogen-free protein preparation that is of storage-quality in liquid state and contains the blood coagulation factor XIIIa stabilized protein preparation that is of storage-quality in liquid state and contains fibrinogen. A chaotropic substance of less than 0.28 mol/liter was added to said protein preparation to avoid or reduce the aggregation of fibrinogen, anda preparation containing thrombin,which are provided in one packaging unit prepared to be used together.Furthermore, stabilized, frozen or lyophilized protein preparations containing fibrinogen and Factor XIII are described. Said protein preparations contain fibrinogen- and Factor XIII which remains stable for more than four weeks after defrosting or reconstitution, as well as one or more added chaotropic substances in a quantity of less than 0.28 mol/liter which prevents or reduces the aggregation of fibrinogen and contain inorganic salts of <100 mmol/liter, preferably <50 mmol/liter.