Systematic evolution of ligands by exponential enrichment: blended selex
US6465189B1 · kind B1 · utility
7Cited by
6References
12Claims
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Key dates
| Filing date | Jun 29, 2000 |
| Grant date | Oct 15, 2002 |
| Priority date | — |
| Expiry date | Feb 26, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1048
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (SEQ ID NO:2). This elastase blended nucleic acid ligand inhibits the biological activity of elastase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.