Thermostable DNA polymerase from carboxydothermus hydrogenoformans

US6468775B1 · kind B1 · utility

Assignee

• Roche Molecular Systems, Inc. · US

Inventors

• Waltraud Ankenbauer · Penzberg, DE
• Ursula Markau · Polling, DE
• Vitaly Svetlichny · Bayreuth, DE
• Gudrun Schmitz-Agheguian · Bernried, DE
• Astrid Reiser · Antdorf, DE
• Bernhard Angerer · Rosenheim, DE
• Christine Ebenbichler · Antdorf, DE
• Frank Laue · Kempten (Allgäu), DE
• Elizaveta Bonch-Osmolovskaya · Moscow, RU

Key dates

• Filing date: Nov 22, 1999
• Grant date: Oct 22, 2002
• Expiry date: Nov 22, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3′-5′ exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.