Antisense intron inhibition of starch branching enzyme expression
US6483009B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 8, 1999 |
| Grant date | Nov 19, 2002 |
| Priority date | — |
| Expiry date | Dec 8, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/111
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for affecting enzymatic activity in a starch producing organism is described. The method comprises expressing in the organism: (a) a first nucleotide which comprises, partially or completely, a first intron of a gene encoding a class A starch branching enzyme in an antisense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron, (b) together with a second nucleotide sequence which comprises, partially or completely, a second intron of a class B starch branching enzyme in an antisense or sense orientation. Also described are antisense sequences, constructs, vectors, transformed cells, and transgenic organisms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.