Subtractive amplification kit useful in the diagnosis of genetic disease mutation or variation
US6498024B1 · kind B1 · utility
15Cited by
11References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 18, 1999 |
| Grant date | Dec 24, 2002 |
| Priority date | — |
| Expiry date | Mar 18, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A kit is provided for preferentially amplifying target RNA in a sample of tester RNA relative to non-target RNA in the sample, the kit driver sequences complementary to the non-target tester RNA under conditions where the driver sequences hybridize to the non-target RNA, a nucleic acid primer capable of hybridizing to the target RNA under conditions suitable for extension of the nucleic acid primer; and a promoter template capable of hybridizing to a DNA that is complementary to the target RNA under conditions suitable for extension of the complementary DNA such that a functional double promoter is formed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.