Patent · US Expired

Miniaturized thermal cycler

US6521447B2 · kind B2 · utility

15Cited by
7References
27Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 3, 2002
Grant dateFeb 18, 2003
Priority date
Expiry dateJul 3, 2022

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01L2400/0688
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

The invention describes a thermal cycler which permits simultaneous treatment of multiple individual samples in independent thermal protocols, so as to implement large numbers of DNA experiments simultaneously in a short time. The chamber is thermally isolated from its surroundings, heat flow in and out of the unit being limited to one or two specific heat transfer areas. All heating elements are located within these transfer areas and at least one temperature sensor per heating element is positioned close by. Fluid bearing channels that facilitate sending fluid into, and removing fluid from, the chamber are provided. The chambers may be manufactured as integrated arrays to form units in which each cycler chamber has independent temperature and fluid flow control. Two embodiments of the invention are described together with a process for manufacturing them.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.