Hydantoinase variants with improved properties and their use for the production of amino acids

US6524837B1 · kind B1 · utility

Assignee

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Frances H. Arnold · Pasadena, US
• Oliver May · Frankfurt, DE
• Karlheinz Drauz · Alzenau, DE
• Andreas Bommarius · Frankfurt, DE

Key dates

• Filing date: Feb 3, 2000
• Grant date: Feb 25, 2003
• Expiry date: Feb 3, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P41/009
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Hydantoinase enzymes which are mutants of a previously isolated hydantoinase having the amino acid SEQ. ID. NO. 2. The mutants include amino acid substitutions at positions 95, 154, 180, 251 and/or 255 of the wild type hydantoinase (SEQ. ID. NO. 2). The mutant hydantoinases, like the parent hydantoinase, are used in the production of optically pure amino acids.