Mammalian cell culture process for producing glycoproteins
US6528286B1 · kind B1 · utility
147Cited by
12References
40Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 26, 1999 |
| Grant date | Mar 4, 2003 |
| Priority date | — |
| Expiry date | May 31, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to novel process for the preparation of glycoproteins by mammalian cell culture wherein the sialic acid content of the glycoprotein recovered is optimized by manipulating the cell culture environment. The invention provides for processes in which the sialic acid content of the glycoprotein recovered is increased by addition of copper ion to a cell culture at a concentration effective for stabilization of the sialic acid content.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.