Complex of biotinylated viral vector and ligand for targeted gene delivery

US6555367B1 · kind B1 · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Sally E. Spence · Frederick, US
• Jeffrey S. Smith · Overland Park, US
• Jonathan R. Keller · Frederick, US

Key dates

• Filing date: Jun 15, 2000
• Grant date: Apr 29, 2003
• Expiry date: Jun 15, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2810/859
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides a composition for targeted delivery of a nucleic acid to a cell comprising a biotinylated recombinant adenovirus, wherein biotin is covalently linked to the recombinant adenovirus, wherein the recombinant adenovirus comprises the nucleic acid, and wherein the recombinant adenovirus is linked via streptavidin to a biotinylated targeting moiety. Also provided by this invention is a method for targeted delivery of a nucleic acid to a selected cell in a subject comprising administering to the subject a composition comprising a biotinylated recombinant adenovirus, wherein biotin is covalently linked to the recombinant adenovirus, wherein the recombinant adenovirus comprises the nucleic acid, and wherein the recombinant virus is linked via streptavidin to a biotinylated targeting moiety that specifically targets the selected cell.