I-Sce I induced gene replacement and gene conversion in embryonic stem cells
US6566579B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 17, 1998 |
| Grant date | May 20, 2003 |
| Priority date | — |
| Expiry date | Jul 17, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/4735
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to methods of introducing a heterologous DNA sequence into a mouse embryonic stem cell wherein the DNA sequence is inserted by homologous recombination into a villin gene/I-SceI hybrid by creating a double strand break with I-SceI meganuclease. Subsequently, the mouse embryonic stem cells can be used to generate a transgenic mouse comprising the heterologous DNA sequence. Additionally, the methods can be used for gene replacement in ovo where a mouse oocyte containing a villin gene/I-SceI hybrid within its genome exists or is first generated. More generally, the methods can be used for the targeted insertion of a heterologous DNA sequence into any cell containing a villin gene/I-SceI hybrid sequence within its genome.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.