Analysis method
US6573053B1 · kind B1 · utility
1Cited by
5References
11Claims
0Family size
Assignee
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Key dates
| Filing date | Sep 24, 2001 |
| Grant date | Jun 3, 2003 |
| Priority date | — |
| Expiry date | Sep 24, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/683
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of comparing genomic DNA from two individuals who share a phenotype, cutting the DNA into fragments long enough to contain on average one or several polymorphisms; combining the fragments under hybridisation conditions and recovering mismatch—free heterohybrids, wherein adapters resistant to nuclease digestion are ligated to the ends of the genomic fragments. A related method uses pooled genomic DNA from individuals with a common phenotype. Another related method uses restricted nucleic acid fragments likely to contain on average less than one natural polymorphism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.