Methods for identifying inhibitors of GADD45 polypeptide activity, and inhibitors of such activity

US6613318B1 · kind B1 · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Xin Wang · Beijing, CN
• Curtis C. Harris · Bethesda, US
• Albert J. Fornace, Jr. · Bethesda, US
• Jill D. Coursen · Boston, US
• Qimin Zhan · Beijing, CN

Key dates

• Filing date: Mar 24, 2000
• Grant date: Sep 2, 2003
• Expiry date: Mar 24, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention is directed to novel methods for assaying for modulators of GADD45 polypeptide activity. The invention also provides means to sensitize a proliferating cell to a DNA base-damaging agent by administration of novel inhibitors of GADD45 polypeptide activity. The invention further provides polypeptides which interfere with the ability of Cdc2/cyclin B1 complexes to cause a pause at the G2/M stage of the cell cycle in response to GADD45, and nucleic acids which encode such polypeptides.