Use of triplex structure DNA in transferring nucleotide sequences

US6682907B1 · kind B1 · utility

Assignee

• INSTITUT PASTEUR · FR

Inventors

• Pierre Charneau · Paris, FR
• Veronique Zennou · Paris, FR
• Hüseyin Firat · Paris, FR

Key dates

• Filing date: Oct 17, 2000
• Grant date: Jan 27, 2004
• Expiry date: Mar 7, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/203
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A recombinant vector comprising a polynucleotide containing a cis-acting central initiation region (cPPT) and a cis-acting termination region (CTS) is disclosed. These regions are of retroviral or retroviral-like origin. The vector further comprises a defined nucleotide sequence (transgene or sequence of interest) and regulatory signals for reverse transcription, expression, and packaging, wherein the regulatory signals are of retroviral or retroviral-like origin. In some embodiments the vector is incorporated into compositions for therapeutic purposes. In other embodiments the vector is incorporated into immunogenic compositions, The vector is also useful for methods such as ex vivo transfection or ex vivo transduction of non-mitotic differentiated cells.