Method for screening for inhibitors and activators of type III secretion machinery in gram-negative bacteria
US6696249B1 · kind B1 · utility
0Cited by
0References
3Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Feb 5, 2001 |
| Grant date | Feb 24, 2004 |
| Priority date | — |
| Expiry date | Feb 5, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to mutant strains of gram-negative bacteria that constitutively secrete proteins via the type III secretion machinery and to methods of identifying molecules that are able to activate or inhibit secretion in wild-type strains of gram-negative bacteria by exposing gram-negative bacterial cells to a sample molecule, wherein the bacterial cells contain a reporter gene transcriptionally fused to a promoter of a gene activated or regulated by the type III secretion machinery and detecting the presence or activity of the product of the reporter gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.