Cytofectin dimers and methods of use thereof
US6696424B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | May 26, 2000 |
| Grant date | Feb 24, 2004 |
| Priority date | — |
| Expiry date | May 26, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/88
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A composition is provided comprising a novel cationic lipid compound having hydrophobic tails and two quaternary ammonium headgroups bridged by a linker. The composition is useful as a cytofectin for facilitating delivery and transfection of biologically active agents, particularly anionic bioactive agents such as DNA, into cells. The composition is useful also as an adjuvant for enhancing the humoral immune response of a vertebrate to an immunogen, especially an immunogen encoded by a polynucleotide-based vaccine. In certain preferred embodiments, the cationic lipid compound is a dimer containing quaternary ammonium headgroups bridged by a linker having DNA and/or cell receptor binding affinity, such as a polypeptide or polyamine. Also disclosed is an immunogenic composition comprising an immunogen and the composition of the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.