Metabolic rate shifts in fermentations expressing recombinant proteins
US6716602B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 1, 2001 |
| Grant date | Apr 6, 2004 |
| Priority date | — |
| Expiry date | Nov 1, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method for increasing product yield of a polypeptide of interest produced by recombinant host cells, where expression of the polypeptide by the recombinant host cells is regulated by an inducible system. More specifically, the method involves culturing the recombinant host cells under conditions of high metabolic and growth rate and then reducing the metabolic rate of the recombinant host cells at the time of induction of polypeptide expression. In particular, the invention provides a method of increasing product yield of an antibody, growth factor, or protease produced by a recombinant E. coli host cell regulated by an inducible system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.