Patent · US Expired

Methods for identifying ligand binding sites in a biomolecule

US6730484B2 · kind B2 · utility

0Cited by
2References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 31, 2002
Grant dateMay 4, 2004
Priority date—
Expiry dateJul 31, 2022

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2500/02
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Methods of identifying ligand binding sites in a target molecule such as a polynucleotide or polypeptide, as well as methods for determining whether a particular site in a target molecule is at or near the ligand binding site, are provided. Ligand binding affinities corresponding to both the target molecule and a modified version thereof are compared, preferably using mass spectral analysis. The modified molecules, or test molecules, incorporate a modification capable of modulating ligand binding affinity when located at or near the ligand binding sites. Binding site information is derived from the known position of the modification.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.