Strand displacement methods employing competitor oligonucleotides for isolating one strand of a double-stranded nucleic acid
US6787310B2 · kind B2 · utility
9Cited by
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25Claims
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Key dates
| Filing date | Jul 18, 2001 |
| Grant date | Sep 7, 2004 |
| Priority date | — |
| Expiry date | Jul 18, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods and kits for isolating one strand of a double-stranded target nucleic acid. The method capitalizes on the differences in the kinetics and thermodynamic stabilities between conventional DNA/DNA, DNA/RNA and RNA/RNA duplexes and heteroduplexes in which one strand of the heteroduplexe is a nucleobase polymer having a net positively charged or net neutral backbone, such as a PNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.