Compositions and methods for random nucleic acid mutagenesis
US6803216B2 · kind B2 · utility
5Cited by
7References
31Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 23, 2002 |
| Grant date | Oct 12, 2004 |
| Priority date | — |
| Expiry date | May 23, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/102
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to compositions and methods for nucleic acid PCR mutagenesis using novel error-prone DNA polymerases and a PCR enhancing factor. The invention also relates to compositions and methods for nucleic mutagenesis with two or more DNA polymerases lacking or exhibiting reduced exonuclease activity. The invention further relates to kit format of said compositions for PCR mutagenesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.