Method for selecting a high affinity, low molecular weight displacer for oligonucleotide purification
US6828436B2 · kind B2 · utility
1Cited by
6References
4Claims
0Family size
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Key dates
| Filing date | May 15, 2002 |
| Grant date | Dec 7, 2004 |
| Priority date | — |
| Expiry date | May 15, 2022 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D15/422
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.