Increasing the digestibility of food proteins by thioredoxin reduction

US6838113B1 · kind B1 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Bob B. Buchanan · Berkeley, US
• Gregorio del Val · Corona, US
• Rosa Lozano · Madrid, ES
• Jin-An Jiao · Fort Lauderdale, US
• Joshua H. Wong · South San Francisco, US
• Boihon C. Yee · Walnut Creek, US

Key dates

• Filing date: Apr 22, 1999
• Grant date: Jan 4, 2005
• Expiry date: Apr 22, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y108/01009
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Thioredoxin, a small dithiol protein, is a specific reductant for major food proteins, allergenic proteins and particularly allergenic proteins present in widely used foods from animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food. Studies showed increased digestion of food and food proteins by pepsin and trypsin following reduction by thioredoxin.