Methods and compositions for HDL holoparticle uptake receptor

US6846636B1 · kind B1 · utility

Assignees

• The American National Red Cross · US
• The United States of America, as represented by the Department of Health and Human Services · US
• MUSC Foundation for Research Development · US

Inventors

• William S. Argraves · Charleston, US
• Samar Hammad · Charleston, US
• Steingrimur Stefansson · Gaithersburg, US
• Bryan Brewer · Potomac, US
• Alan T. Remaley · Bethesda, US

Key dates

• Filing date: May 13, 1999
• Grant date: Jan 25, 2005
• Expiry date: May 13, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/705
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides an isolated mammalian receptor which specifically binds a high density lipoprotein holoparticle, comprising a subunit of approximately 45-600 kDa molecular weight and one or more subunits selected from the group consisting of a subunit of approximately 40-50 kDa molecular weight, a subunit of approximately 120 kDa molecular weight and a subunit of approximately 400 kDa molecular weight. In addition, the present invention provides a method of screening a substance for the ability to modulate the HDL holoparticle binding and/or internalization activity of the receptor of this invention, comprising: a) contacting the substance with a cell producing a functional HDL receptor; and b) assaying the cell for a modulation of the HDL holoparticle binding and/or internalization activity of the receptor, whereby a modulation of the HDL holoparticle binding and/or internalization activity of the receptor identifies a substance with the ability to modulate the HDL holoparticle binding and/or internalization activity of the ADL receptor.