Methods, compositions, and cells for encapsidating recombinant vectors in AAV particles
US6893865B1 · kind B1 · utility
26Cited by
70References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 27, 2000 |
| Grant date | May 17, 2005 |
| Priority date | — |
| Expiry date | Apr 27, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2750/14152
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Isolated recombinant polynucleotides comprising elements which promote encapsidation into AAV particles, packaging cells comprising the recombinant polynucleotides, and methods for their use are provided in the present invention. These isolated recombinant polynucleotides comprise a non-AAV ITR encapsidation element (such as the P1 sequence located within the AAV S1 integration site of human chromosome 19) operably linked to one or more heterologous genes to be encapsidated. The constructs may be either integrated into a mammalian cell genome, maintained episomally, or provided transiently.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.