Patent · US Expired

Fluorescence intensity multiple distributions analysis: concurrent determination of diffusion times and molecular brightness

US6965113B2 · kind B2 · utility

0Cited by

6References

19Claims

0Family size

Assignee

EVOTEC AG · DE

Inventors

Peet Kask · Tallinn, EE
Stefan Jäger · Hamburg, DE
Kaupo Palo · Haabneeme, EE
Ulo Mets · Tallinn, EE
Karsten Gall · Lunestedt, DE

Key dates

Filing date	Feb 9, 2001
Grant date	Nov 15, 2005
Priority date	—
Expiry date	Sep 30, 2021

Classification

Technology area (CPC G)Physics
CPC primaryG01N2021/6463
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A method for distinguishing samples having flourescent particles includes (a) monitoring intensity fluctuations of fluorescence emitted by the particles in at least one measurement volume by detecting sequences of photon counts by at least one photon detector, (b) determining, from the sequences of photon counts, intermediate statistical data involving at least two probability functions of the number of photon counts detected in different sets of counting time intervals, and (c) determining from the intermediate statistical data a distribution of particles as a function of at least two arguments, wherein one argument is a specific brightness of the particles, or a measure thereof, and another argument is a diffusion coefficient of the particles, or a measure thereof.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.