Gold implantation/deposition of biological samples for laser desorption three dimensional depth profiling of tissues
US6989528B2 · kind B2 · utility
23Cited by
5References
34Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 4, 2004 |
| Grant date | Jan 24, 2006 |
| Priority date | — |
| Expiry date | Jun 15, 2024 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J49/0418
- WIPO fieldElectrical machinery, apparatus, energy
- WIPO sectorElectrical engineering
Abstract
The present invention enhances the laser desorption of biological molecular ions from surfaces by creating a surface localized MALDI particle matrix by ion implantation of low energy ionized clusters (gold, aluminum, etc.) or chemically derivatized clusters into the near surface region of the sample. MALDI analysis of the intact biomolecules on the surface or within a narrow subsurface region defined by the implantation range of the ions can then be performed by laser desorption into a mass spectrometer or, in a preferred embodiment, into a combined ion mobility orthogonal time of flight mass spectrometer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.