Methods for identifying inhibitors of GADD45 polypeptide activity and inhibitors of such activity

US7005419B1 · kind B1 · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Xin Wang · Beijing, CN
• Curtis C. Harris · Bethesda, US
• Albert J. Fornace, Jr. · Bethesda, US
• Jill D. Coursen · Boston, US
• Qimin Zhan · Beijing, CN

Key dates

• Filing date: Jun 20, 2003
• Grant date: Feb 28, 2006
• Expiry date: Nov 19, 2023

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention is directed to novel methods for assaying for modulators of GADD45 polypeptide activity. The invention also provides means to sensitize a proliferating cell to a DNA base-damaging agent by administration of novel inhibitors of GADD45 polypeptide activity. The invention further provides polypeptides which interfere with the ability of Cdc2/cyclin B1 complexes to cause a pause at the G2/M stage of the cell cycle in response to GADD45, and nucleic acids which encode such polypeptides.