Patent · US Expired

Method for improving the stability of linear DNA in cell-free in vitro transcription/translation systems

US7011958B2 · kind B2 · utility

1Cited by

3References

6Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Manfred Watzele · Weilheim, DE
Thomas Hoffmann · Santa Cruz, US
Cordula Nemetz · Wolfratshausen, DE
Dieter Heindl · Munich, DE
Thomas Metzler · Munich, DE
Wolfgang Mutter · Bernried, DE

Key dates

Filing date	Apr 17, 2002
Grant date	Mar 14, 2006
Priority date	—
Expiry date	Sep 20, 2022

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/68
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.