Thermostable enzyme promoting the fidelity of thermostable DNA polymerases-for improvement of nucleic acid synthesis and amplification in vitro

US7030220B1 · kind B1 · utility

Assignees

• Roche Diagnostics GMBH · DE
• Roche Molecular Systems, Inc. · US

Inventors

• Waltraud Ankenbauer · Penzberg, DE
• Frank Laue · Kempten (Allgäu), DE
• Harald Sobek · Penzberg, DE
• Michael Greif · Penzberg, DE

Key dates

• Filing date: Sep 27, 2000
• Grant date: Apr 18, 2006
• Expiry date: Jan 25, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A purified thermostable enzyme is derived from the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3′–5′ exonuclease and cleaves to produce 5′-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Tag especially for nucleic acid amplification by the polymerase chain reaction (PCR).