Process of purifying phosphoramidites
US7030230B2 · kind B2 · utility
15Cited by
19References
73Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 25, 2002 |
| Grant date | Apr 18, 2006 |
| Priority date | — |
| Expiry date | Sep 3, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H19/04
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A process of purifying phosphoramidite precursors useful in inter alia synthesis of oligonucleotides comprises dissolving a crude phosphoramidite in a polar phase, adding a basic compound to the polar phase, adding a portion of water to the polar phase, contacting the polar phase with a first apolar phase to extract impurity into the apolar phase, separating the first apolar phase from the polar phase, adding a second aliquot of water to the polar phase, and contacting the polar phase with a second apolar phase, whereby the phosphoramidite partitions into the second apolar phase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.