Method for testing hormonal effects of substances

US7074566B2 · kind B2 · utility

Assignee

• SCHERING CORPORATION · US

Inventors

• Siegmund Wolf · Bad Klosterlausnitz, DE
• Maik Obendorf · Weimar, DE
• Rene Meyer · Atherton, US
• Jens Schroeder · Kiel, DE

Key dates

• Filing date: Jun 6, 2003
• Grant date: Jul 11, 2006
• Expiry date: Jan 21, 2024

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/74
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The method for testing of substances for hormonal effects, especially for androgenic or anti-androgenic effects, includes exposing cells transfected with two vectors to the substances, wherein one vector contains a DNA, which codes for a nuclear receptor protein, or a fragment thereof, especially a human nuclear receptor protein, or a fragment thereof, and the other vector contains a DNA, which codes for the HSRNAAM co-modulator, or a fragment thereof; and measuring transcription activity, which the nuclear receptor protein, or its fragment, activates or releases in the presence of the HSRNAAM co-modulator, or its fragment, and/or measuring the influence of the substance on the interaction between the nuclear receptor protein, or its fragment, and the HSRNAAM co-modulator, or its fragment, by protein-protein interaction or by protein-protein-DNA interaction. Also a method for determining interference in the co-modulation mechanism between androgen receptor protein and HSRNAAM co-modulator is described.