Methods and compositions for detecting larval Taenia solium with a cloned diagnostic antigen

US7094576B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Victor C. W. Tsang · Decatur, US
• Ryan Greene · Austin, US
• Patricia P. Wilkins · Gainesville, US
• Kathy Hancock · Atlanta, US

Key dates

• Filing date: Mar 30, 2001
• Grant date: Aug 22, 2006
• Expiry date: Aug 2, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/805
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Compositions and methods for the detection of Taenia solium and the diagnosis of T. solium infection are described. The nucleotide and amino acid sequences of the antigenic T. solium polypeptides gp50a, gp50b and gp50c are provided. The compositions contain synthetic antigenic polypeptides of larval origin prepared using the sequences described herein. Probes and primers for the detection or amplification of T. solium nucleic acid molecules are also described. The polypeptides can be administered to a human or animal to protect against T. solium infection. In addition, the polypeptides are useful as research tools for studying T. solium and as reagents in assays for the detection of T. solium antibodies in a biological sample. The methods are sensitive and specific assays that utilize the stable recombinant or synthetic antigenic polypeptides or nucleic acid molecules encoding the larval polypeptides.