Production of pseudotyped recombinant AAV virions

US7094604B2 · kind B2 · utility

Assignee

• University of Florida Research Foundation, INC. · US

Inventors

• Richard Snyder · Gainesville, US
• Sergei Zolotukhin · Gainesville, US
• Yoshihisa Sakai · Gainesville, US
• Barry J. Byrne · Baltimore, US
• Mark R. Potter · Gainesville, US
• Irine Zolotukhin · Gainesville, US
• Scott Loiler · Columbus, US
• Vince Chiodo · Gainesville, US
• Nicholas Muzyczka · Gainesville, US
• William W. Hauswirth · Gainesville, US
• Terence Flotte · Holden, US
• Corinna Burger · Gainesville, US
• Edgardo Rodriguez · Gainesville, US
• Kevin Nash · Seffner, US
• Thomas Fraites · Ringwood, US

Key dates

• Filing date: Mar 11, 2004
• Grant date: Aug 22, 2006
• Expiry date: Mar 11, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Vectors that encode Adeno-Associated Virus (AAV) Rep and Cap proteins of different serotypes and Adenovirus transcription products that provide helper functions were used to produce pseudotyped recombinant AAV (rAAV) virions. Purification methods generated pseudotyped rAAV virion stocks that were 99% pure with titers of 1×1012–1×1013 vector genomes/ml.