Utilization of non-viral sequences for minus-strand DNA transfer and gene reconstitution

US7101541B2 · kind B2 · utility

Assignee

• The United States of America as Represented by the Secretary of the Department of Health and Human Services · US

Inventors

• Wei Hu · 红钢城街道, CN
• Vinay K. Pathak · Morgantown, US

Key dates

• Filing date: May 15, 2001
• Grant date: Sep 5, 2006
• Expiry date: Sep 27, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/203
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A retroviral vector for gene reconstitution is provided that includes a 3′ portion of a heterologous nucleic acid sequence 5′ of a first att site and a 5′ portion of the heterologous nucleic acid sequence 3′ of a second att site. A sub-portion of the 3′ portion of the heterologous nucleic acid sequence and a sub-portion the 5′ portion of the heterologous nucleic acid sequence are direct repeats. A retroviral vector for gene reconstitution is also provided that includes a 3′ portion of a heterologous nucleic acid sequence inserted into or adjacent to a 5′ retroviral terminal repeat of the retroviral vector, and a 5′ portion of the heterologous nucleic acid sequence inserted into or adjacent to a 3′ retroviral terminal repeat of the retroviral vector, wherein the 3′ and the 5′ retroviral terminal repeats each comprise an att site. Methods and kits are also provided.