Method for creating polynucleotide and polypeptide sequences
US7166466B2 · kind B2 · utility
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106References
25Claims
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Assignee
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Key dates
| Filing date | Feb 19, 2003 |
| Grant date | Jan 23, 2007 |
| Priority date | — |
| Expiry date | Apr 12, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1034
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.