Systematic evolution of ligands by exponential enrichment: blended SELEX
US7176295B2 · kind B2 · utility
5Cited by
15References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 2, 2002 |
| Grant date | Feb 13, 2007 |
| Priority date | — |
| Expiry date | Jan 20, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1048
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (SEQ ID NO:2). This elastase blended nucleic acid ligand inhibits the biological activity of elastase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.