Use of alpha factor sequences in yeast expression systems
US7198919B1 · kind B1 · utility
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Key dates
| Filing date | May 24, 1995 |
| Grant date | Apr 3, 2007 |
| Priority date | — |
| Expiry date | May 24, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/555
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The isolation of the yeast α-factor genes is described. The promoter and signal peptide portions are isolated and joined to DNA coding for proteins heterologous to yeast in a plasmid which is used to transform yeast cells. The yeast expresses the heterologous DNA and processes and secretes the heterologous protein.
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