Automatic threshold setting and baseline determination for real-time PCR
US7228237B2 · kind B2 · utility
24Cited by
4References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 20, 2002 |
| Grant date | Jun 5, 2007 |
| Priority date | — |
| Expiry date | May 20, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06F2218/08
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
The invention discloses a system and methods for quantitating the presence of nucleic acid sequences by evaluation of amplification data generated using real-time PCR. In one aspect, the methods may be adapted to identify a threshold and threshold cycle for one or more reactions based upon evaluation of exponential and baseline regions for each amplification reaction. The methodology used in the analysis may be readily automated such that subjective user interpretation of the data is substantially reduced or eliminated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.