Production of novel Newcastle disease virus strains from cDNAs and improved live attenuated Newcastle disease vaccines

US7244558B1 · kind B1 · utility

Assignees

• University of Maryland · US
• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Siba K. Samal · College Park, US
• Peter L. Collins · Kensington, US

Key dates

• Filing date: May 5, 2000
• Grant date: Jul 17, 2007
• Expiry date: May 5, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/18161
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention concerns cDNAs for making attentuated, infectious Newcastle disease virus (NDV). Another aspect of the invention relates to methods of making the cDNAs. Another aspect of the invention is a vector containing the cDNA optionally linked to an operable promoter. Within the scope of the invention are vaccines comprising the attenuated, infectious NDV. Also disclosed are methods of making the vaccines and methods of using the vaccines to prevent or treat Newcastle disease in an avian host. The present invention also concerns the nucleotide sequences of the entire genome of NDV, the leading region, the trailing region, and the NP region, as well as proteins encoded by these nucleotide sequences.