Method of etching islands of cells in a grid pattern
US7276367B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 14, 2004 |
| Grant date | Oct 2, 2007 |
| Priority date | — |
| Expiry date | Nov 18, 2024 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T83/0304
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus and process for monitoring migratory cell proliferation with restricted migration on a substrate includes providing a substrate, coating the substrate with extracellular matrix, plating cells suspended in cell culture media on extracellular matrix, and placing intersecting channels across the extracellular matrix components by removing the extracellular matrix components from the channels to isolate islands of the extracellular matrix components on the substrate. When the cells are immersed with a fluid, migration of the cells is confined to the isolated islands of the extracellular matrix components, permitting long-term observation of a migratory population.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.