Isolation tool for viable c-kit expressing cells
US7285413B2 · kind B2 · utility
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6Claims
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Key dates
| Filing date | Dec 12, 2002 |
| Grant date | Oct 23, 2007 |
| Priority date | — |
| Expiry date | Jun 12, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A tool for the isolation of c-kit expressing cells is provided. This tool consists of a c-kit plasmid targeting vector which is capable of integrating into the wild type c-kit allele and encodes a chimeric fluorescent protein comprising a nucleolar localization signal such as, TCOF-12, RLP313, RPS254 or Fxr2h5. The construct generates a condensed, bright, fluorescent signal that can be localized in living tissue and after dissociation. The construct allows visualization using confocal microscopy and allows automated cell sorting of the dissociated cells using amongst others flow cytometry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.