Substrates for isolating, reacting and microscopically analyzing materials
US7297497B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 8, 2006 |
| Grant date | Nov 20, 2007 |
| Priority date | — |
| Expiry date | May 8, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54366
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is enhanced by the intensity calibration spots. (420) to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment. Methods of forming layers of the device include controlled drawing from a bath of co…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.