Detection of nucleic acid sequence variations using phase Mu transposase
US7316903B2 · kind B2 · utility
81Cited by
14References
31Claims
0Family size
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Key dates
| Filing date | Mar 26, 2004 |
| Grant date | Jan 8, 2008 |
| Priority date | — |
| Expiry date | Apr 20, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates, e.g., to a method of detecting a mismatch in a double stranded nucleic acid target, comprising (a) contacting the target with (i) a Mu-end nucleic acid, and (ii) a phage Mu transposase, under conditions effective for the Mu-end nucleic acid to transpose into the target at about the site of a mismatch, if the target comprises a mismatch, and (b) detecting transposition of the Mu-end DNA into the target, wherein transposition of the Mu-end nucleic acid into the target at a predominant site indicates the presence of a mismatch at that site.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.