Patent · US Expired

System of components for preparing oligonucleotides

US7321828B2 · kind B2 · utility

177Cited by
201References
4Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 28, 1998
Grant dateJan 22, 2008
Priority date
Expiry dateApr 28, 2018

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG16B30/00
  • WIPO fieldComputer technology
  • WIPO sectorElectrical engineering

Abstract

Interative, preferably computer based iterative processes for generating synthetic compounds with desired physical, chemical and/or bioactive properties, i.e., active compounds, are provided. During iterations of the processes, a target nucleic acid sequence is provided or selected, and a library of candidate nucleobase sequences is generated in silico according to defined criteria. A “virtual” oligonucleotide chemistry is chosen and a library of virtual oligonucleotide compounds having the selected nucleobase sequences is generated. These virtual compounds are reviewed and compounds predicted to have particular properties are selected. The selected compounds are robotically synthesized and are preferably robotically assayed for a desired physical, chemical or biological activity. Active compounds are thus generated and, at the same time, preferred sequences and regions of the target nucleic acid that are amenable to oligonucleotide or sequence-based modulation are identified.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.