Production of fucosylated carbohydrates by enzymatic fucosylation synthesis of sugar nucleotides; and in situ regeneration of GDP-fucose

US7335500B2 · kind B2 · utility

Assignee

• THE SCRIPPS RESEARCH INSTITUTE · US

Inventors

• Chi-Huey Wong · Village of La Jolla, US
• Yoshitaka Ichikawa · San Diego, US
• Gwo-Jenn Shen · Carlsbad, US
• Kun Liu · Edison, US

Key dates

• Filing date: Nov 19, 2001
• Grant date: Feb 26, 2008
• Expiry date: Sep 13, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/26
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.