Nucleic acids encoding fusion peptides

US7348423B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Eva Hoess · Starnberg, DE
• Thomas Meier · Cherbourg, FR
• Gabriele Pestlin · Birkenfeld, DE
• Friedrich Popp · Penzberg, DE
• Klaus Reichert · Oberhausen, DE
• Rainer Schmuck · Benediktbeuern, DE
• Bernd Schneidinger · Starnberg, DE
• Christoph Seidel · Weilheim, DE
• Wilhelm Tischer · Peißenberg, DE

Key dates

• Filing date: Oct 20, 2004
• Grant date: Mar 25, 2008
• Expiry date: Nov 23, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16122
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10,…