Physical mapping method using molecular combing technique allowing positioning of a great number of clones within a genome
US7368234B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 11, 2003 |
| Grant date | May 6, 2008 |
| Priority date | — |
| Expiry date | Dec 7, 2023 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for detecting or locating one or several genes of one or several specific A DNA sequence or one or several molecules reacting with DNA on a B DNA where the method can include: (a) fixing and combing a certain amount of the B DNA on a combing surface; (b) reacting the product of step (a) with one or several probes, linked with the gene(s) or specific A DNA sequences, or with the molecules capable of reacting with DNA; (c) extracting information corresponding to at least one of the following categories: (1) the position of the probes, (2) the distance between the probes, (3) the size of the probes (the total sum of sizes for quantifying the number of hybridized probes) and determining from this extracted information the presence, the location and/or the amount of a gene(s) or specific A DNA sequences. This method can be used in particular for the diagnosis of genetic diseases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.