Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
US7399619B2 · kind B2 · utility
14Cited by
9References
35Claims
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Key dates
| Filing date | May 24, 2005 |
| Grant date | Jul 15, 2008 |
| Priority date | — |
| Expiry date | May 24, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.