Thermostable enzyme promoting the fidelity of thermostable DNA polymerases—for improvement of nucleic acid synthesis and amplification in vitro

US7410782B2 · kind B2 · utility

Assignees

• Roche Diagnostics GMBH · DE
• Roche Molecular Systems, Inc. · US

Inventors

• Waltraud Ankenbauer · Penzberg, DE
• Frank Laue · Kempten (Allgäu), DE
• Harald Sobek · Penzberg, DE
• Michael Greif · Penzberg, DE

Key dates

• Filing date: Sep 29, 2005
• Grant date: Aug 12, 2008
• Expiry date: Sep 29, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A purified thermostable enzyme is derived form the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3′-5′ exonuclease and cleaves to produce 5′-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Taq especially for nucleic acid amplification by the polymerase chain reaction (PCR).