Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use
US7435570B2 · kind B2 · utility
11Cited by
17References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 11, 2004 |
| Grant date | Oct 14, 2008 |
| Priority date | — |
| Expiry date | Aug 19, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/0077
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.