Method for preparing botulinum neurotoxin type A light chain
US7465457B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 14, 2006 |
| Grant date | Dec 16, 2008 |
| Priority date | — |
| Expiry date | Apr 14, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/33
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a preparation of botulinum toxin light chain type A or E, wherein the preparation is both catalytically active and soluble. Preferably, the preparation consists essentially of amino acid residues 1 through 425 of the botulinum toxin light chain type A. A method of screening inhibitors is also provided, wherein the method comprises exposing a test inhibitor to the preparation of botulinum toxin light chain type A and evaluating the biological activity of the preparation. In another embodiment, a method of providing a catalytically active, soluble preparation of botulinum toxin light chain, type A is provided, wherein the method comprises obtaining an expression vector comprising a DNA sequence encoding amino acid residues 1-425 and expressing a polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.